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Image Search Results
Journal: MethodsX
Article Title: Optimizing lncRNA-miRNA interaction analysis: Modified crosslinking and immunoprecipitation (M-CLIP) assay
doi: 10.1016/j.mex.2024.103028
Figure Lengend Snippet: Workflow of the Modified Crosslinking and Immunoprecipitation (M-CLIP) Assay This flowchart summarizes the key steps of the M-CLIP assay for analyzing lncRNA-miRNA interactions. Cells are harvested and crosslinked using formaldehyde, followed by lysis and immunoprecipitation of Ago2-bound complexes. Crosslinking is reversed, and RNA is isolated and analyzed by qRT-PCR to detect target lncRNA-miRNA interactions, specifically UCA1-let-7 in this study.
Article Snippet: Quantitative Real-Time PCR (qRT-PCR) for the candidate lncRNA and miRNAs • Materials Needed : UCA1 qRT-PCR primers ( ) Let-7
Techniques: Modification, Immunoprecipitation, Lysis, Isolation, Quantitative RT-PCR
Journal: MethodsX
Article Title: Optimizing lncRNA-miRNA interaction analysis: Modified crosslinking and immunoprecipitation (M-CLIP) assay
doi: 10.1016/j.mex.2024.103028
Figure Lengend Snippet: List of Let-7 Primers.
Article Snippet: Quantitative Real-Time PCR (qRT-PCR) for the candidate lncRNA and miRNAs • Materials Needed : UCA1 qRT-PCR primers ( ) Let-7
Techniques: Sequencing
Journal: MethodsX
Article Title: Optimizing lncRNA-miRNA interaction analysis: Modified crosslinking and immunoprecipitation (M-CLIP) assay
doi: 10.1016/j.mex.2024.103028
Figure Lengend Snippet: qRT-PCR analysis of Ago2 bound UCA1 and let-7 miRNAs. CLIP assay was performed and qRT-PCR was carried out to detect Ago-2-bound UCA1 as well as let-7 miRNAs along with respective IgG control. The fold enrichments of UCA1 bound to Ago2 as well as let-7 miRNAs bound to Ago2 were calculated over the IgG controls, are presented graphically. Error bars indicate mean ± SEM. Each of the presented experiments was repeated thrice.
Article Snippet: Quantitative Real-Time PCR (qRT-PCR) for the candidate lncRNA and miRNAs • Materials Needed : UCA1 qRT-PCR primers ( ) Let-7
Techniques: Quantitative RT-PCR, Control